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. 2021 Jun 17;10(6):765. doi: 10.3390/pathogens10060765

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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HuscFvs-mediated neutralization of LasB elastolytic activity. (A,B) The results of fluorogenic substrate assay shown as plots of elastase activity of LasB (negative neutralization control), nLasB after treatment with different concentrations of HuscFv-N42 (A) and HuscFv-N45 (B), compared to nLasB in Buffer (baseline) and nLasB treated with 5 mM EDTA (positive neutralization control). ACTIVITY (RFU), relative fluorescence units of the enzymatic activity per minute. NS, not significantly different. (C,D) are the results of elastin-Congo Red assay for determining HuscFvs-mediated neutralization of the nLasB-elastolyticity. The bar graphs of average (mean ± SD) of OD 495 nm of three independent experiments are shown. Elastolytic activities of the nLasB after treatment with various concentrations of HuscFv-N42 (C) and HuscFv-N45 (D), compared with that of LasB alone (negative neutralization control) and nLasB treated with 5 mM EDTA (positive neutralization control). NS, not significantly different.