Figure 3.

Retarded seed germination and early seedling growth in ipa1-NIL seeds is caused by GA defects. (A) The contents of bioactive GAs in the seeds after 48 h imbibition. Values are means ±SE (n = 3). (B) The relative expression level of OsGAMYB at different time points. Values are means ±SE (n = 3). (C–E) Germination rate of NILs soaked in 0 μM (C), 10 μM (D), and 30 μM (E) GA₃ solution. Values are means ±SE (n = 3). More than 50 seeds were measured in each replicate. (F,G) The shoot (F) and root (G) lengths of seedlings grow on filter paper soaked with water or GA₃ solution. Values are means ±SE (n = 36). Significant differences were determined using Student’s t-test (* p < 0.05, ** p < 0.01).