Figure 1.

LH pulses profile in gonad-intact WT and Tac1KO mice. LH samples were collected every 10 minutes for 180 minutes from gonad-intact WT and Tac1KO male mice. (A, B) are 2 representative examples of pulsatile LH secretion in WT (A) and Tac1KO  (B) male mice. Pulses number/180 min (C), Basal LH (D), LH pulse amplitude (E) and total secretory mass assessed by area under the curve (AUC) (F) were all significantly different in Tac1KO vs WT mice. ****P = 0.0001, Student t test. *represents LH pulses following the protocol described in the methods.