Figure 2.

Validation of RNA-seq data ex vivo and in vivo. (A) mRNA expression of representative genes in colonic organoids from WKY and SHR rats by qPCR (n = 5 per group). (B) mRNA levels of RT1-CE5 and RT1-Ba by qPCR in the proximal colons of SHR and WKY rats (n = 6 per group). Fold changes relative to WKY group. GADPH expression was used for normalization. Values are means ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001, unpaired t test or Mann–Whitney U test. (C) Protein levels of MHC II class gene, RT1-A in organoids and proximal colons of WKY and SHR rats by Western blot. β-actin was used as a reference protein.