Figure 1.

Macrophage-based ADCC assay. (A) Increasing numbers of MDCK or Raji target cells were incubated with D-luciferin substrate and luminescence was measured at 1 h post-addition. (B) MDCK-cH6/1 and Raji-cH6/1 cells were stained with HA-specific antibody 8H9 followed by anti-mouse Alexa 555. (C) MDCK target cells were incubated with 6F12 or isotype control Ab in the absence or presence of various ratios of macrophages and luminescence was measured at the indicated time points. No killing was observed in the absence of antigen expression. (D) MDCK-cH6/1 target cells were incubated as in C. Killing was observed in the presence of antigen expression and the specific antibody. (E) Raji target cells were incubated with the indicated antibodies. Killing was again observed with the specific antibody.