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. 2021 Jun 18;13(6):2087. doi: 10.3390/nu13062087

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of exosomes derived from fisetin-treated keratinocytes on the mitochondria in HFSCs. (A) TOMM20⁺ cells in HFSCs were detected by immunocytochemistry using anti-TOMM antibody. The number of TOMM20⁺ cells (red in pie chart) was determined using IN Cell Analyzer 2200. (B) Mitochondria in HFSCs were detected by immunocytochemistry using specific probes (MitoTracker Red CMXRos and MitoTracker Green FM). The relative number of cells harboring active mitochondria (yellow cells in photo; red in pie chart) was determined using IN Cell Analyzer 2200.