Figure 3.

Bergenin attenuated anxiety-like behavior in 5xFAD Tg mice and exhibited no change in locomotor activity. (A) Time spent in center (s), (B) Number of line crossings, and (C) Number of rearing were evaluated in the open field test (OFT) for all the experimental groups. Results were expressed as mean ± S.D (n = 10). * p < 0.05, ** p < 0.01 and *** p < 0.001 versus the 5xFAD Tg mice group, ## p < 0.01 and ### p < 0.001 versus the control (wild-type). (D) Diagrammatic representation of the hippocampal (HC) region in the mouse brain. FT-IR spectra of the HC region of mice brain in wild-type, 5xFAD Tg mice, and Bergenin groups in (E) 4000–500 cm⁻¹ (Full spectra), (F) 3600–3100 cm⁻¹ (Amide-A region), (G) 1800–1500 cm⁻¹ (Ester C=O, Amide-I, and Amide-II region), (H) 3050–2800 cm⁻¹ (olefinic =C-H, symmetric and asymmetric CH3, and CH2 regions), and (I) 1300–1000 cm⁻¹ (symmetric and asymmetric PO2- region).