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. 2021 Jun 21;10(6):987. doi: 10.3390/antiox10060987

Figure 6.

Figure 6

Effect of GENI on autophagy in yeast. (a) Fluorescent images of YOM38 yeast containing plasmid pR316-GFP-Atg8 after treatment of RES or GENI observed with a two-photon confocal fluorescent microscope. (b) The percentage of YOM38 cells containing free-GFP (green). Seven pictures containing more than 60 cells in each group were used for statistical analysis. (c) Western blot analysis of GFP-Atg8 and free GFP in yeast after treatment with RES or GENI for 22 h in SD medium. (d) The digital results of (c). (e) Western blot analysis results for the GFP-Atg8 and free GFP in yeast after treatment with RES or 1 µM GENI in time course. (f) The digital results of (e). (g,h) The replicative lifespan of atg2 and atg32 of yeast after treatment of RES at 10 µM and GENI at 1 µM. Experiments were repeated thrice, and the data are presented as means ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 represent significant difference compared with the control group.