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. 2021 Jun 19;22(12):6591. doi: 10.3390/ijms22126591

Figure 3.

Figure 3

Activation of THP-1 cells by IL-4 or IL-10 increases FXIII-A activity which is primarily associated with the cell cytoplasm and membrane fractions. THP-1 cells (6 × 10⁶ cells/mL) left unstimulated or stimulated with 20 ng/mL IL-4, 20 ng/mL IL-10 or 100 ng/mL LPS for 24 h. The supernatant was collected by centrifugation of cells at 2500× g for 5 min. The cells were washed twice in cold sterile PBS and centrifuged at 2500× g for 5 min before being resuspended in RIPA buffer. The lysate was centrifuged at 14,000× g for 15 min and the supernatant was collected as cytoplasm fractions and the precipitate was resuspended in RIPA buffer as cell membrane fractions. FXIII-A activity (IU/mL) was measured in (A) total cell lysate of unstimulated or IL-4, IL-10 or LPS-stimulated cells and cell fractions (B) supernatant, (C) cytoplasm or (D) membrane. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. unstimulated THP-1 cells. Data are expressed as the mean with standard error of the mean SEM (n = 3).