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. 2021 Jun 19;22(12):6591. doi: 10.3390/ijms22126591

Figure 5.

Figure 5

FXIII-A deficient thrombi are stabilized by THP-1 cells and human-derived monocytes stimulated with IL-4 and IL-10. Model thrombi were formed with pooled normal plasma (PNP) or FXIII-/- plasma in the presence of and FITC-labelled fibrinogen under arterial shear rates. Lysis was subsequently induced by tPA (1 μg/mL) and monitored for 4 h. Fluorescence release is directly proportional to the degree of fibrinolysis. PNP thrombi were formed in the absence (grey) and presence (orange) of the TG inhibitor and FXIII-/- thrombi formed without (black) and with (blue) 3 × 105/mL (A) THP-1 cells or (D) human-derived monocytes. *** p < 0.001 vs. PNP; # p < 0.05 vs. FXIII-/--deficient thrombi. (B) THP-1 cells or (E) human-derived monocytes were stimulated for 24 h with IL-4 (pink) or IL-10 (purple) prior to thrombus formation # p < 0.05 or *** p < 0.001 vs. FXIII-/--deficient thrombi (C) THP-1 cells or (F) human-derived monocytes were stimulated for 24 h with LPS (green) before addition to thrombi. # p < 0.05 vs. FXIII-/--deficient thrombi. Values represent the mean ± SEM, n = 4.