Figure 3.

Illustrative plot of intracellular Cal AM accumulation versus time in A549 (A), HepG2 (B) and SW620 (C) cells treated with Ag20 in the presence and absence of verapamil. Verapamil-dependent calcein AM accumulation in A549 (D), HepG2 (E) and SW620 (F) cells treated for 2, 6 and 24 h with 50 μg/cm³ Ag20 expressed as the slope of the rectilinear segment of the fluorescence increment over time. One-way ANOVA and Dunnett’s post hoc test were used. Significance level: ** p < 0.01; *** p < 0.001. Data expressed as arithmetic mean ± SD; n = 3.