Figure 2.

Effect of ρ- chlorophenylalanine (PCPA, a serotonin synthesis inhibitor) pre-treatment on cardamonin against mechanical allodynia of CCI-induced neuropathic pain mice were evaluated using von-Frey filament test. Data were presented as mean ± SEM (n = 6). The caret, hash, and asterisks denote the significance levels (one-way ANOVA, followed by Tukey’s post hoc test. ^{^} p < 0.05 as compared to the sham, ^{^^} p < 0.01 as compared to sham, ^## p < 0.01, ^### p < 0.001 as compared to vehicle and *** p < 0.001 as compared to cardamonin-treated group. SHM (Sham); VEH (Vehicle, 10 mL/kg i.p.); C (Cardamonin, 10 mg/kg i.p.); AMI (Amitriptyline, 20 mg/kg i.p.); PC (ρ- chlorophenylalanine, 100 mg/kg i.p.).