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. 2021 Jun 16;22(12):6466. doi: 10.3390/ijms22126466

Figure 2.

Figure 2

Figure 2

The effect of isookanin on cell migration in PGE2-induced HMEC-1 cells. Cells were pretreated with the indicated concentrations of isookanin for 2 h before stimulation with PGE2 (20 µM) or VEGF (100 ng/mL) for 24 h. (A) Cell migration was measured using the scratch migration assay, and cells were photographed under a microscope; scale bars are 80 µm. (B) Cell migration was measured using the transwell migration assay, and cells were photographed under a microscope; scale bars are 200 µm. (C) Cells were extracted with 10% acetic acid, the absorbance at 600 nm was used to calculate the percentage of migrated cells. The results are mean ± standard deviation (SD) (n = 3). # p < 0.05 vs. control. * p < 0.05 vs. PGE2-treated control.