Figure 6.

Effects of isookanin on the phosphorylation of ERK and CREB in PGE₂-induced HMEC-1 cells. The HMEC-1 cells were cultured for 24 h and then treated with PGE₂ (20 µM) in the presence or absence of isookanin. Cell lysates were prepared at 20 min and were then subjected to Western blot analysis. (A) The bands for phospho-ERK1/2 and phospho-CREB were detected and normalized to their total forms of ERK1/2 and CREB. (B) The scanning densitometric values of each band were analyzed with Image J. The results are mean ± standard deviation (SD) (n = 3). ^# p < 0.05, ^### p < 0.001 vs. control. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. PGE₂-treated control.