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. Author manuscript; available in PMC: 2021 Aug 4.
Published in final edited form as: Cell Metab. 2020 Jun 8;32(2):243–258.e6. doi: 10.1016/j.cmet.2020.05.017

Figure 1. Adipose tissue iNKT cells lose PLZF expression with age.

Figure 1.

(a) Representative histogram of PLZF expression in adipose iNKT cells from neonatal and adult mice (n = 3–5 histograms analyzed per group).

(b) Quantification of PLZF expression in splenic and adipose iNKT cells from neonatal and adult mice (n = 3–5 mice or pooled mice per group). Red line indicates the baseline PLZF mean fluorescence intensity of PLZFNEG T cells.

(c) Adipose tissue iNKT cells in neonatal and adult mice were analyzed by biaxial gating of PLZF against either Tbet or RORγT (n = 8 mice).

(d) Schematic diagram outlining adoptive transfer experiment.

(e) Expression of PLZF in transferred splenic iNKT cells recovered from recipient spleen and adipose tissue (n = 5 mice).

NS, not significant (P > 0.05); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Two-tailed Student’s t-test. Error bars indicate mean (± S.E.M.). Data are representative of three or more independent experiments (a-c) or two experiments (e).