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. Author manuscript; available in PMC: 2021 Aug 4.
Published in final edited form as: Cell Metab. 2020 Jun 8;32(2):243–258.e6. doi: 10.1016/j.cmet.2020.05.017

Figure 3. Exposure to adipose tissue lipids induces regulatory iNKT cells.

Figure 3.

(a) Expression of Nfil3 in scRNA-seq clusters (left) or in cells from scRNA-seq experiment segregated based on Klrb1c (NK1.1) expression (right).

(b) Expression of Nfil3 within fine-clustered NKT1-B cells.

(c) E4BP4 expression in splenic iNKT cells adoptively transferred into congenically marked mice and recovered from spleen and adipose tissue one week later (n = 5–6 organs per group).

(d) Percentage of IL-10+ donor iNKT cells recovered from spleen and adipose tissue after αGalCer stimulation (n = 9 organs per group).

(e) Expression of E4BP4 in splenic iNKT cells cultured in control media or in the presence of adipose tissue. (n = 2 technical replicates per group).

(f) Expression of E4BP4 in donor splenic iNKT cells recovered from recipient spleen and adipose tissue (n = 9 organs per group).

(g) Expression of E4BP4 in iNKT cells cultured in control media or adipose-conditioned media.

(h) E4BP4 expression in iNKT cells exposed to adipose-conditioned media left unfractionated or fractionated into aqueous and organic phases (n = 3 technical replicates per group).

Error bars indicate mean (± S.E.M.). NS, not significant (P > 0.05); *P < 0.05; **P < 0.01; ***P < 0.001. Two-tailed student’s t-test (c, d, e, f). Data representative of (c) or combined from (d, f) two independent experiments or representative of three or more experiments (e, g, h).