KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| TotalSeq™-A0301 anti-mouse Hashtag 1 Antibody | Biolegend | Ca#155801 |
| TotalSeq™-A0302 anti-mouse Hashtag 2 Antibody | Biolegend | Ca#155803 |
| Ultra-LEAF™ Purified anti-mouse IFN-γ Antibody | Biolegend | Ca#505834 |
| anti-mouse/human CD11b (M1/70) | Biolegend | Ca#101242 |
| anti-mouse CD45 (30-F11) | Biolegend | Ca#103138 |
| anti-mouse TCRβ (H57–597) | Biolegend | Ca#109224 |
| anti-mouse NKp46 (29A1.4) | Biolegend | Ca#137612 |
| anti-mouse PLZF (Mags.21F7) | Thermofisher | Ca#53-9320-82 |
| anti-mouse T-bet (4B10) | Thermofisher | Ca#45-5825-82 |
| anti-mouse RORγT (B2D) | Thermofisher | Ca#25-6981-82 |
| anti-mouse E4BP4 (S2M-E19) | Thermofisher | Ca#12-5927-82 |
| anti-mouse CD69 (FN50) | Thermofisher | Ca#25-0699-41 |
| anti-mouse Nur77 (12.14) | Thermofisher | Ca#53-5965-82 |
| anti-mouse TNF (MP6-XT22) | Thermofisher | Ca#25-7321-82 |
| anti-mouse IL-17A (eBio17B7) | Thermofisher | Ca#45-7177-82 |
| anti-mouse IFN-γ (XMG1.2) | Thermofisher | Ca#53-7311-82 |
| anti-mouse IL-2 (JES6–5H4) | Thermofisher | Ca#25-7021-82 |
| anti-mouse IL-10 (JES5–16E3) | Thermofisher | Ca#12-7101-41 |
| anti-mouse IL-4 (11B11) | Thermofisher | Ca#25-7041-82 |
| anti-mouse IL-13 (eBio13A) | Thermofisher | Ca#12-7133-82 |
| anti-mouse F4/80 (BM8) | Thermofisher | Ca#17-4801-82 |
| anti-mouse CD19 (ID3) | Thermofisher | Ca#47-0193-82 |
| anti-mouse NK1.1 (PK136) | Thermofisher | Ca#25-5941-82 |
| anti-mouse CD3e (500A2) | Thermofisher | Ca#HM3420 |
| anti-mouse CD11c (N418) | Thermofisher | Ca#47-0114-82 |
| anti-mouse CD301 (ER-MP23) | Thermofisher | Discontinued |
| anti-mouse CD107a (eBio1D4B) | Thermofisher | Ca#12-1071-82 |
| anti-mouse Granzyme B (NGZB) | Thermofisher | Ca#25-8898-82 |
| anti-mouse CD4 (RM4–5) | Thermofisher | Ca#48-0042-82 |
| anti-mouse CD8a (53–6.7) | Thermofisher | Ca#11-0081-82 |
| anti-mouse ST2 (RMSST2–2) | Thermofisher | Ca#17-9335-82 |
| anti-mouse Foxp3 (FJK-16s) | Thermofisher | Ca#11-5773-82 |
| anti-mouse CD45.2 (104) | Thermofisher | Ca#45-0454-82 |
| anti-mouse CD45.1 (A20) | Thermofisher | Ca#25-0453-82 |
| anti-mouse Ki67 (SolA15) | Thermofisher | Ca#11-5698-82 |
| anti-mouse Siglec-F (E50–2440) | BD Biosciences | Ca#565526 |
| PE Mouse anti-XBP-1S | BD Biosciences | Ca#562642 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Alpha-galactosylceramide (KRN7000) | Avanti | Ca#867000 |
| Recombinant murine IL-2 | Peprotech | Ca#212–12 |
| Recombinant murine IL-7 | Peprotech | Ca#217–17 |
| Palmitic acid | Sigma | Ca#P0500 |
| 4μ8c | Sigma | Ca#SML0949 |
| Sodium phenylbutyrate | Sigma | Ca#SML0309 |
| Tunicamycin | Sigma | Ca#T7765 |
| Chloroform | Sigma | Ca#288306 |
| Methanol | Sigma | Ca#34860 |
| Monensin solution, 1000X | Biolegend | Ca#420701 |
| CellTracker™ Green CMFDA Dye | Thermofisher | Ca#C2925 |
| Annexin V | Thermofisher | Ca#88-8007-72 |
| Brefeldin A | Thermofisher | Ca#00-4506-51 |
| LipidTox Green | Thermofisher | Ca#H34475 |
| Trizol Reagent | Thermofisher | Ca#15596026 |
| FoxP3 staining kit | Thermofisher | Discontinued |
| Cell stimulation cocktail | Thermofisher | Ca#00-4970-03 |
| 7AAD | BD Biosciences | Ca#559925 |
| Cytofix/Cytoperm | BD Biosciences | Ca#554714 |
| Collagenase Type 2 | Worthington | Ca#LS004176 |
| ACK Lysing Buffer | VWR | Ca#10128–802 |
| Ultrapure LPS, E. coli 0111:B4 | InvivoGen | Ca#tlrl-3pelps |
| Critical Commercial Assays | ||
| FAM-FLIVO Kit | ImmunoChemistry Technologies | Ca#980 |
| BRDU Flow Kit | BD BioSciences | Ca#559619 |
| RNeasy Mini Kit | Qiagen | Ca#74104 |
| Brilliant III SYBR Green | Agilent | Ca#600883 |
| Deposited Data | ||
| Raw scRNAseq Data | GEO | GSE142845 |
| Raw data (non-RNAseq) | Mendeley Data | doi: 10.17632/2d6ywgb8bj.1 |
| Experimental Models: Cell Lines | ||
| Primary murine iNKT cell line | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Vα14 TN | Stephanie Dougan Lab (Clancy-Thompson, et al., 2018) | N/A |
| Mouse: CD1d1floxed | Richard Blumberg Lab (Olszak, et al., 2014) | N/A |
| Mouse: B6.129S6-Tcra-Jtm1Tgi/J (Jα18 KO) | Mark Exley Lab | N/A |
| Mouse: B6.129S6-Cd1d1/Cd1d2tm1Spb/J (CD1d KO) | Mark Exley Lab | N/A |
| Mouse: C57BL/6J (WT) | Jackson Laboratories | Ca#000664 |
| Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) | Jackson Laboratories | Ca#002014 |
| Mouse: B6.129S7-Ifngtm1Ts/J (IFNγ KO) | Jackson Laboratories | Ca#002287 |
| Mouse: B6.129S6-Il10tm1Flv/J (TIGER) | Jackson Laboratories | Ca#008379 |
| Mouse: B6;FVB-Tg(Adipoq-cre)1Evdr/J (AdipoQ Cre) | Jackson Laboratories | Ca#010803 |
| Oligonucleotides | ||
| qPCR Primers (18s, Nfil3, Il10, Xbp1 total, Xbp1s, Hprt, Nos2, Il6, Arg1, Il13, Il4 | Integrated DNA Technologies | See Table S3 |
| Software and Algorithms | ||
| ImageJ | ImageJ | www.imagej.net |
| Adiposoft package | ImageJ | www.imagej.net/Adiposoft |
| FlowJo | Treestar | https://www.flowjo.com/ |
| R | The R Project for Statistical Computing | www.r-project.org |
| Graphpad Prism 6 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| Mouse PBS57:CD1d Tetramer | NIH Tetramer Core Facility/Emory Vaccine Center | N/A |
| Standard Fat (Chow) Diet | Pico Labs | Ca#0007688 |
| High Fat Diet (60% kcal) | Research Diets, Inc | Ca#D12492 |
| Blood Glucose Meter | McKesson | Ca#960302 |
| Blood Glucose Test Strips | McKesson | Ca#960298 |