Skip to main content
. 2021 Jun 17;10(6):1525. doi: 10.3390/cells10061525

Figure 2.

Figure 2

Normal differentiation and morphology of CAP2-KO neurons. (A) Immunoblots confirmed absence of CAP2 from CAP2-KO brain and unchanged expression levels of CAP1 in CAP2-KO brains. GAPDH was used as the loading control. Graph showing CAP1 and CAP2 levels normalized to the loading control, GAPDH, with CTR set to 1 in CTR and CAP2-KO brain lysates. Black circles: normalized CAP1 and CAP2 levels in three biological replicates. Green circles and error bars: MV and SEM. (B) Representative hippocampal neurons from CTR and CAP2-KO mice at differentiation stages 1 to 3 [31]. Neurons were stained with an antibody against doublecortin (Dcx, magenta), the DNA maker Hoechst (blue), and phalloidin (green). (C) Stage distribution for CTR and CAP2-KO neurons after five hours in vitro (HIV5) and at DIV1 and DIV2. Number of (D) primary neurites and (E) neurite endpoints in stage 2 CTR and CAP2-KO neurons at DIV1 and DIV2. (F) Endpoints/neurites ratio in stage 2 CTR and CAP2-KO neurons at DIV1 and DIV2. (G) Neurite width and (H) total neurite length in stage 2 CTR and CAP2-KO neurons. Scale bar (in µm): 10 (B). n.s.: p ≥ 0.05, * p < 0.05. Error bars represent SEM.