Table 1.
Fetal blood gases, pH and metabolites during the baseline period, asphyxia and recovery.
| Group | Baseline | 17 min UCO | +1 h | +4 h | +24 h | +72 h | +96 h | +120 h | +144 h | +168 h |
|---|---|---|---|---|---|---|---|---|---|---|
| pH | ||||||||||
| Sham asphyxia | 7.36 ± 0.01 | 7.36 ± 0.00 | 7.37 ± 0.00 | 7.37 ± 0.01 | 7.35 ± 0.00 | 7.35 ± 0.01 | 7.35 ± 0.00 | 7.34 ± 0.01 | 7.35 ± 0.01 | 7.35 ± 0.01 |
| Asphyxia + vehicle | 7.36 ± 0.01 | 6.81 ± 0.01 * | 7.30 ± 0.00 * | 7.36 ± 0.02 | 7.37 ± 0.01 | 7.36 ± 0.01 | 7.36 ± 0.01 | 7.36 ± 0.01 | 7.35 ± 0.01 | 7.36 ± 0.01 |
| Asphyxia + GDQ | 7.38 ± 0.01 | 6.82 ± 0.01 * | 7.32 ± 0.01 * | 7.39 ± 0.01 | 7.37 ± 0.01 | 7.37 ± 0.01 | 7.37 ± 0.01 | 7.37 ± 0.01 * | 7.37 ± 0.00 * | 7.37 ± 0.01 |
| PaCO2 (mmHg) | ||||||||||
| Sham asphyxia | 52.4 ± 1.2 | 51.2 ± 1.5 | 54.3 ± 1.3 | 52.7 ± 1.4 | 55.8 ± 1.3 | 54.4 ± 1.3 | 51.9 ± 1.6 | 54.7 ± 1.4 | 55.3 ± 2.2 | 53.0 ± 1.8 |
| Asphyxia + vehicle | 51.4 ± 1.1 | 150.7 ± 2.5 * | 48.9 ± 1.2 * | 51.9 ± 1.1 | 50.1 ± 1.9 * | 50.3 ± 1.6 | 50.5 ± 1.6 | 52.3 ± 1.6 | 51.6 ± 1.7 | 51.3 ± 2.2 |
| Asphyxia + GDQ | 50.9 ± 1.2 | 147.0 ± 2.3 * | 48.6 ± 1.3 * | 49.6 ± 0.7 | 49.2 ± 1.5 * | 47.5 ± 1.5 * | 47.9 ± 1.4 | 48.6 ± 1.0 * | 49.7 ± 0.6 * | 49.2 ± 1.8 |
| PaO2 (mmHg) | ||||||||||
| Sham asphyxia | 28.9 ± 1.4 | 27.7 ± 1.2 | 29.0 ± 1.1 | 28.6 ± 1.7 | 28.8 ± 1.8 | 29.6 ± 1.3 | 28.3 ± 2.1 | 28.4 ± 0.7 | 27.6 ± 1.0 | 28.9 ± 0.9 |
| Asphyxia + vehicle | 26.8 ± 0.7 | 5.8 ± 0.4 * | 31.8 ± 2.6 | 25.7 ± 1.6 | 29.9 ± 2.0 | 31.8 ± 1.8 | 30.7 ± 2.2 | 28.6 ± 1.8 | 29.1 ± 1.8 | 28.7 ± 2.1 |
| Asphyxia + GDQ | 28.3 ± 1.1 | 5.7 ± 0.4 * | 31.9 ± 1.3 | 27.3 ± 1.4 | 30.9 ± 1.4 | 35.0 ± 1.8 | 33.6 ± 1.4 | 31.2 ± 1.3 | 31.0 ± 1.2 | 29.5 ± 1.3 |
| Lactate (mmol/L) | ||||||||||
| Sham asphyxia | 0.8 ± 0.0 | 0.7 ± 0.0 | 0.8 ± 0.0 | 2.5 ± 0.5 | 0.8 ± 0.0 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.9 ± 0.1 |
| Asphyxia + vehicle | 0.9 ± 0.1 | 6.8 ± 0.1 * | 4.4 ± 0.2 * | 3.8 ± 0.5 | 1.4 ± 0.2 * | 1.0 ± 0.1 | 0.9 ± 0.1 | 0.9 ± 0.1 | 0.9 ± 0.1 | 0.9 ± 0.1 |
| Asphyxia + GDQ | 0.8 ± 0.0 | 6.5 ± 0.3 * | 4.0 ± 0.1 * | 3.0 ± 0.6 | 1.1 ± 0.1 | 0.7 ± 0.0 # | 0.7 ± 0.0 # | 0.7 ± 0.0 # | 0.7 ± 0.0 | 0.7 ± 0.0 |
| Glucose (mmol/L) | ||||||||||
| Sham asphyxia | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.4 ± 0.1 | 1.6 ± 0.1 | 1.2 ± 0.0 | 1.4 ± 0.1 | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.4 ± 0.1 |
| Asphyxia + vehicle | 1.3 ± 0.1 | 0.7 ± 0.1 * | 1.7 ± 0.1 * | 1.5 ± 0.1 | 1.5 ± 0.1 | 1.4 ± 0.1 | 1.4 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.3 ± 0.0 |
| Asphyxia + GDQ | 1.3 ± 0.1 | 0.7 ± 0.2 * | 1.7 ± 0.1 * | 1.6 ± 0.1 | 1.5 ± 0.2 | 1.5 ± 0.1 | 1.4 ± 0.1 | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.4 ± 0.1 |
Data are presented as mean ± SEM. Abbreviations: GDQ, Gardiquimod; UCO, umbilical cord occlusion; PaCO2, arterial pressure of carbon dioxide; PaO2, arterial pressure of oxygen. Statistical significance was determined by one-way ANOVA, followed by Fisher’s least significant difference post hoc analysis. * p < 0.05 vs. sham asphyxia; # p < 0.05 vs. asphyxia + vehicle.