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. 2021 Jun 18;9(6):1325. doi: 10.3390/microorganisms9061325

Table 3.

Prevention of Colorectal cancer (CRC) using various prebiotics in cell lines.

Reference Type of Study Prebiotic Probiotic Mechanism of Action
[31] Research
Cell growth-inhibitory
activity in DLD-1 cells and
WirDr cells		 fructo-oligosaccharides (FOS) Bifidobacterium longum (BB536-y)
  • ✓

    Intake of BB536-y with FOS was associated with a higher Bifidobacterium detection rate than that of BB536-y alone.

  • ✓

    The contents of butyric acid, isobutyric acid, and acetic acid, namely of SCFA, were also decreased.

  • ✓

    Analysis of the results of culture of DLD-1 cells and WirDr cells in the presence of butyric acid, isobutyric acid, and acetic acid revealed that each of the substances showed significant cell growth-inhibitory activity, with the activity being the highest for butyric acid, followed by that for isobutyric acid and acetic acid.
[32] Research
HT29 Cell line		 Soluble dietary fibre extracted from plantain inflorescence (PIF) 1
  • ✓

    Promoted the growth of L. casei and B. bifidum which indirectly inhibits the growth of pathogenic strains and protects the intestine.

  • ✓

    Rich in SCFA particularly that obtained from fermentation by B. bifidum.

  • ✓

    The study particularly identified up-regulation of one of the key apoptotic inducing protein—Apoptosis-inducing factor, mitochondria-associated. Thus, it is evident from the experimental results that the fermentation supernatant contains SCFA which induces ROS mediated apoptosis in HT29 cells.
[33] Research
Cell lines and MTT assay 2 		Polysaccharide fraction from mushrooms Cantharellus cibarius The prebiotic potential was revealed in relation to Lactobacillus strains. Crude polysaccharides were found to inhibit the proliferation of colon cancer cells with the simultaneous absence of toxicity towards normal cells.

1 Rich source of dietary fiber and polyphenols exhibit anticancer potential in HT29 colon cancer cell. 2 Antiproliferative activity (MTT assay). Cell proliferation was assessed by means of the MTT assay, in which the yellow tetrazolium salt (MTT) is metabolized by viable cells to purple formazan crystals.