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. 2021 Jun 17;10(6):1234. doi: 10.3390/plants10061234

Figure 7.

Figure 7

Effect of PC on p-JNK, p-ERK, and TNF-α proteins in RAW 264.7 cells. (A) The expression levels of TNF-α, p-ERK, and p-JNK proteins were measured by performing Western blotting analysis. The Raw 264.7 cells were treated with PC for 24 h. 1—Normal group, 2—LPS-treated control group, 3—LPS-treated control group + 10 µg/mL PC, 4—LPS-treated control group + 100 µg/mL PC, 5—LPS-treated control group + 200 µg/mL PC, 6—Normal group + 10 µg/mL PC, 7—Normal group + 100 µg/mL PC, 8—Normal group + 200 µg/mL PC. Quantitative analysis of the results of the Western blotting analysis are shown in (B) for p-JNK, in (C) for p-ERK, and in (D) for TNF-α proteins. β-Actin was used as an internal control. Values are mean ± SEM (n = 3). These values are expressed as the percentage of the control tested protein/β-actin for each sample. p-JNK (B): 1 vs. 2 p < 0.0001, 2 vs. 3 p = 0.0001, 2 vs. 4 p = 0.0014, 2 vs. 5 p < 0.0146; p-ERK (C): 1 vs. 2 p = 0.0001, 2 vs. 3 p = 0.0002, 2 vs. 4 p < 0.0001, 2 vs. 5 p < 0.0001, 1 vs. 6 p < 0.0001, 1 vs. 7 p < 0.0001, 1 vs. 8 p < 0.0001; TNF-α (D): 1 vs. 2 p = 0.0006, 2 vs. 3 p = 0.0004, 2 vs. 4 p = 0.0057, 2 vs. 5 p = 0.0066. (one-way ANOVA for multiple comparisons, **p < 0.01, ***p < 0.001; +p < 0.05, ++p < 0.01, +++p < 0.001).