Table 1.

Main goals of the IHD-EPITRAN study with brief respective descriptions. IHD, Ischemic heart disease.

Goal	Description
To identify IHD-specific candidate biomarkers and lay the foundation for both clinical and diagnostic efficacy studies in the future	Corss-sectional cohort study design Comparsion of blood RNA modifications of IHD cohorts to non-IHD cohorts Porspective cohort study design Comparsion of circulating RNA modifications of IHD-positive cohorts to thoes in IHD-negative cohort
To establish protocals and modify detection methods and workflows for RNA modications	Optimization of RNA isolation, type-fragmentation for modification-targeted RNA sequencing using both second and third generation methodologies
To increase the pathophysiologic knowledge of IHD	The cross-sectional and prospective comparsions of blood and right atrial appendage epitramscriptomes is expectable to provide novel insight to the IHD pathophysiology
To open venues for therapeutic development previously been disregarded due to the lack of research and methodological limitations	Identification of novel epotanscriptomic candidate blood biomarkers for IHD can provide potenial targets also for future drug development