Figure 4.

VIP-modulated complement system proteins. Secreted levels of (a,c) C1r and (b,d) C3 were determined by ELISA and Multiplex, respectively, in the secretomes from SF and AC alone and in co-culture (n = 6). Data are presented as mean ± SEM of triplicate determinations. (a,b) Constitutive protein expression of (a) C1r and (b) C3 PTX3 in the secretomes from SF and AC alone and in co-culture at 48 h. Results are presented as pg corrected by the number of cells for each condition. (c,d) Protein expression of (c) C1r and (d) C3 in the SF-AC co-cultures secretomes at 48 h of treatment with and without 10 nM 45 kDa Fn-fs in the presence and absence of 10 nM VIP. ** p < 0.01 Fn-fs+VIP vs. Fn-fs.