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. 2021 Jun 16;22(12):6441. doi: 10.3390/ijms22126441

Figure 5.

Figure 5

VIP-modulated ECM degradation proteins. Secreted levels of (a,d) DCN, (b,e) CTSB, and (c,f) MMP2 were determined by ELISA and Multiplex, respectively, in the secretomes from SF and AC alone and in co-culture (n = 6). Data are presented as mean ± SEM of triplicate determinations. (a–c) Constitutive protein expression of (a) DCN, (b) CTSB, and (c) MMP2 in the secretomes from SF and AC alone and in co-culture at 48 h. * p < 0.05, ** p < 0.01, *** p < 0.001. Results are presented as pg corrected by the number of cells for each condition. (d–f) Protein expression of (d) DCN, (e) DCN, and (f) MMP2 in the SF-AC co-cultures secretomes at 48 h of treatment with and without 10 nM 45 kDa Fn-fs in the presence and absence of 10 nM VIP. * p < 0.05, *** p < 0.001 Fn-fs+VIP vs. Fn-fs.