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Experimental design of SF and AC co-cultures and isolated cultures for ELISA and Multiplex analysis. (a) Basal conditions in the SF-AC transwells. (b) Treatments of SF-AC co-cultures. (c) Treatments of SF and AC isolated cultures. SF, synovial fibroblasts; AC, articular chondrocytes; Fn-fs, fibronectin fragments; VIP, vasoactive intestinal peptide.
