Table 1.
Proteins identified by SILAC analysis in the secretome of OA SF-AC co-cultures.
| Acc No a | Protein Symbol | Protein Name | Biological Function |
|---|---|---|---|
| P01023 | A2MG | Alpha-2-macroglobulin | Immune response |
| P63261 | ACTG | Actin, cytoplasmic 2 | Cytoskeletal protein |
| P12814 | ACTN1 | Alpha-actinin-1 | Cytoskeletal protein |
| P04075 | ALDOA | Fructose-bisphosphate aldolase A | Cellular metabolic process |
| P15144 | AMPN | Aminopeptidase N | Immune response |
| P01008 | ANT3 | Antithrombin-III | Angiogenesis |
| P27695 | APEX1 | DNA-(apurinic or apyrimidinic site) lyase | Oxidative stress |
| Q9P1U1 | ARP3B | Actin-related protein 3B | Cytoskeletal organization |
| P61769 | B2MG | Beta-2-microglobulin | Immune response |
| Q15582 | BGH3 | Transforming growth factor-beta-induced protein ig-h3 | TGF-beta signaling |
| P00736 | C1R | Complement C1r subcomponent | Complement activation |
| P09871 | C1S | Complement C1s subcomponent | Complement activation |
| P40121 | CAPG | Macrophage-capping protein | Macrophage function |
| P07858 | CATB | Cathepsin B | ECM degradation |
| P07339 | CATD | Cathepsin D | ECM degradation |
| P07711 | CATL1 | Cathepsin L1 | ECM degradation |
| P16070 | CD44 | CD44 antigen | ECM degradation/Inflammatory response |
| P00751 | CFAB | Complement factor B | Complement activation |
| P08603 | CFAH | Complement factor H | Complement activation |
| P36222 | CH3L1 | Chitinase-3-like protein 1 | Inflammatory response |
| P10909 | CLUS | Clusterin | Immune response/Complement activation |
| Q8N137 | CNTRB | Centrobin | Centriole duplication |
| P08123 | CO1A2 | Collagen alpha-2(I) chain | ECM component |
| P01024 | CO3 | Complement C3 | Complement activation |
| P12109 | CO6A1 | Collagen alpha-1(VI) chain | ECM component |
| P12111 | CO6A3 | Collagen alpha-3(VI) chain | ECM component |
| Q99715 | COCA1 | Collagen alpha-1(XII) chain | ECM component |
| P49747 | COMP | Cartilage oligomeric matrix protein | ECM component |
| Q14019 | COTL1 | Coactin-like protein | Cytoskeletal protein binding |
| P02511 | CRYAB | Alpha-crystallin B chain | Cytoskeletal protein binding |
| O94985 | CSTN1 | Calsyntenin-1 | Cytoskeletal protein binding |
| P13639 | EF2 | Elongation factor 2 | Cytoskeletal protein binding |
| P06733 | ENOA | Alpha-enolase | Immune response |
| Q12805 | FBLN3 | EGF-containing fibulin-like extracellular matrix protein 1 | ECM component/Negative regulator of chondrocyte differentiation |
| P02751 | FINC | Fibronectin | ECM component/ECM degradation |
| Q06828 | FMOD | Fibromodulin | ECM component |
| P04406 | G3P | Glyceraldehyde-3-phphate dehydrogenase | Cellular metabolic process/Immune response |
| P06744 | G6PI | Gluce-6-phphate isomerase | Cellular metabolic process/Immune response |
| P50395 | GDIB | Rab GDP dissociation inhibitor beta | Immune response |
| P07093 | GDN | Glia-derived nexin | ECM component/Serine protease inhibitor |
| P28161 | GSTM2 | Glutathione S-transferase Mu 2 | Cellular metabolic process/Inflammatory response |
| P09211 | GSTP1 | Glutathione S-transferase P | Cellular metabolic process/Inflammatory response |
| P57053 | H2BFS | Histone H2B type F-S | Immune response |
| P02042 | HBD | Hemoglobin subunit delta | Oxygen transport |
| P11142 | HSP7C | Heat shock cognate 71 kDa protein | Immune response |
| P04792 | HSPB1 | Heat shock protein beta-1 | Immune response |
| P17936 | IBP3 | Insulin-like growth factor-binding protein 3 | Cell proliferation and differentiation |
| P22692 | IBP4 | Insulin-like growth factor-binding protein 4 | Cell proliferation and differentiation |
| P24592 | IBP6 | Insulin-like growth factor-binding protein 6 | Cell proliferation and differentiation |
| Q16270 | IBP7 | Insulin-like growth factor-binding protein 7 | Cell proliferation and differentiation |
| P05155 | IC1 | Plasma protease C1 inhibitor | Complement activation |
| P0DOX7 | IGK | Immunoglobulin kappa light chain | Immune response |
| P05231 | IL6 | Interleukin-6 | Inflammatory response |
| P13645 | K1C10 | Keratin, type I cytoskeletal 10 | Cytoskeletal protein |
| P35527 | K1C9 | Keratin, type I cytoskeletal 9 | Cytoskeletal protein |
| P04264 | K2C1 | Keratin, type II cytoskeletal 1 | Cytoskeletal protein |
| P14618 | KPYM | Pyruvate kinase PKM | Cellular metabolic process/Immune response |
| P00338 | LDHA | L-lactate dehydrogenase A chain | Cellular metabolic process |
| P17931 | LEG3 | Galectin-3 | Inflammatory response |
| P51884 | LUM | Lumican | ECM component |
| P33908 | MA1A1 | Mannosyl-oligosaccharide 1,2-alpha-mannidase IA | Cellular metabolic process |
| P40925 | MDHC | Malate dehydrogenase, cytoplasmic | Cellular metabolic process |
| P14174 | MIF | Macrophage migration inhibitory factor | Immune response |
| P03956 | MMP1 | Interstitial collagenase | ECM degradation |
| P08253 | MMP2 | 72 kDa type IV collagenase | ECM degradation |
| P08254 | MMP3 | Stromelysin-1 | ECM degradation |
| P26038 | MOES | Moesin | Cytoskeletal protein binding |
| P22392 | NDKB | Nucleoside diphosphate kinase B | Immune response |
| Q96TA1 | NIBL1 | Niban-like protein 1 | Apoptosis suppression |
| P05121 | PAI1 | Plasminogen activator inhibitor 1 | Serine protease inhibitor |
| Q15113 | PCOC1 | Procollagen C-endopeptidase enhancer 1 | Cellular metabolic process |
| P30101 | PDIA3 | Protein disulfide-isomerase A3 | Cellular metabolic process |
| Q15084 | PDIA6 | Protein disulfide-isomerase A6 | Cellular metabolic process |
| P18669 | PGAM1 | Phosphoglycerate mutase 1 | Cellular metabolic process |
| P00558 | PGK1 | Phosphoglycerate kinase 1 | Cellular metabolic process |
| P21810 | PGS1 | Biglycan | ECM component/ECM degradation |
| P07585 | PGS2 | Decorin | ECM component/ECM degradation |
| O60664 | PLIN3 | Perilipin-3 | Protein transport |
| P62937 | PPIA | Peptidyl-prolyl cis-trans isomerase A | Cellular metabolic process |
| P23284 | PPIB | Peptidyl-prolyl cis-trans isomerase B | Cellular metabolic process |
| Q06830 | PRDX1 | Peroxiredoxin-1 | Immune response |
| P30041 | PRDX6 | Peroxiredoxin-6 | Immune response |
| Q92954 | PRG4 | Proteoglycan 4 | ECM component/Control of synovial growth and adhesion of to the cartilage surface |
| P07737 | PROF1 | Profilin-1 | Cytoskeletal protein binding |
| P49721 | PSB2 | Proteasome subunit beta type-2 | Immunoproteasome assembly |
| Q9UL46 | PSME2 | Proteasome activator complex subunit 2 | Immunoproteasome assembly |
| P26022 | PTX3 | Pentraxin-related protein 3 | Inflammatory response |
| O00391 | QSOX1 | Sulfhydryl oxidase 1 | Oxidation-reduction process |
| P55017 | S12A3 | Solute carrier family 12 member 3 | Inflammatory response |
| P50454 | SERPH | Serpin H1 | ECM organization |
| Q9H299 | SH3L3 | SH3 domain-binding glutamic acid-rich-like protein 3 | Oxidation-reduction process |
| P04179 | SODM | Superoxide dismutase [Mn], mitochondrial | Oxidation-reduction process |
| P09486 | SPRC | SPARC | Cell proliferation and differentiation |
| P42224 | STAT1 | Signal transducer and activator of transcription 1-alpha/beta | Immune response |
| P23381 | SYWC | Tryptophan--tRNA ligase, cytoplasmic | Angiogenesis |
| P68363 | TBA1B | Tubulin alpha-1B chain | Cytoskeletal protein |
| Q13509 | TBB3 | Tubulin beta-3 chain | Cytoskeletal protein |
| P07437 | TBB5 | Tubulin beta chain | Cytoskeletal protein |
| P24821 | TENA | Tenascin | ECM component |
| P01033 | TIMP1 | Metalloproteinase inhibitor 1 | Metalloprotease inhibitor |
| P16035 | TIMP2 | Metalloproteinase inhibitor 2 | Metalloprotease inhibitor |
| P29401 | TKT | Transketolase | Oxidation-reduction process |
| P60174 | TPIS | Triosephosphate isomerase | Oxidation-reduction process |
| P02787 | TRFE | Serotransferrin | Iron binding transport protein |
| P02788 | TRFL | Lactotransferrin | Iron binding transport protein |
| Q8NBS9 | TXND5 | Thioredoxin domain-containing protein 5 | Immune response |
| P19971 | TYPH | Thymidine phosphorylase | Angiogenesis |
| P22314 | UBA1 | Ubiquitin-like modifier-activating enzyme 1 | Proteasome degradation |
| O60701 | UGDH | UDP-glucose 6-dehydrogenase | Biosynthesis of ECM components |
| Q6EMK4 | VASN | Vasorin | TGF-beta signaling |
| P08670 | VIME | Vimentin | Cytoskeletal protein/Immune response |
| P18206 | VINC | Vinculin | ECM binding/Immune response |
| P02774 | VTDB | Vitamin D-binding protein | Vitamin D transport and storage |
| P04004 | VTNC | Vitronectin | ECM binding/Immune response |
| Q5GH72 | XKR7 | XK-related protein 7 | XK related family |
a Protein accession number according to the SwissProt and TrEMBL databases. SILAC, stable isotope labeling by amino acids in cell culture; OA, osteoarthritis; SF, synovial fibroblasts; AC, articular chondrocytes.