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. 2021 Jun 21;10(6):1562. doi: 10.3390/cells10061562

Figure 9.

Figure 9

The NF-κB p65 nuclear translocation in LX-2 cells. (A) Incubation of LX-2 cells (2 × 106/well) with lipopolysaccharide (LPS, 100 ng/mL) and with a serial dose of eritoran (0–10 μg/mL) for 6 h and 24 h. Western blotting of nuclear and cytoplasmic protein of NF-κB p65 was performed (n = 4/group). (B) Cell viability determined by the methyl thiazolyl tetrazolium (MTT) assay in LX-2 cells (1 × 104/well) incubated with the control medium (Ctrl), LPS (100 ng/mL) and a serial concentration of eritoran (0–10 μg/mL) for 24 h (n = 8/group); # p < 0.05 vs. the Ctrl group; * p < 0.05 vs. the group treated with LPS and without eritoran.