Figure 1.

(a) Separation of mono- and disaccharides on Waters BEH Amide column with mobile phases containing 0.1% NH₄OH; The elution was achieved using gradient elution at 170 µL/min flow rate. Solvents were: A—MilliQ + 0.1% NH₄OH, B—MeCN + 0.1% NH₄OH. Gradient program was: 0.0–10 min linear ramp 0.1–60% A, 10.01–25.00 hold at 0.1% A. The detection was performed in Single-Ion-Reaction (SIR) mode (b) Separation of mono- and disaccharides on Waters BEH HILIC column with mobile phases containing neat solvents. The elution was achieved using gradient elution at 170 µL/min flow rate. Solvents were: A—MilliQ, B—MeCN. Gradient program was: 0.0–20 min linear ramp 0.1–40% A, 20.01–30.00 hold at 0.1% A. The detection was performed in Single-Ion-Reaction (SIR) mode at a concentration level of 10 µg/mL for all saccharides.