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Band shift assay of the interaction between (A) G5D, (B) Au:G5D, (C) G5D:FA, (D) Au:G5D:FA, and mRNA. Incubation mixtures (20 µL) in HBS contained varying amounts of the nanoparticle preparation and 0.05 µg FLuc-mRNA corresponding to w/w ratios of 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, and 8:1 in lanes 2–8, respectively (A–D). Lane 1: naked mRNA control. Arrows indicate endpoint ratios.
