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. 2021 Jun 21;9(6):680. doi: 10.3390/vaccines9060680

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Three different kinds of microbeads used: Regular beads admixed with CCs and OCs; The acid or alkali treated regular beads with OC only, (iii) beads restricted with CC. The microbeads were characterized using W6/32 (IgG2a) bound to peptide-associated and peptide-free CCs, but not bound to the OCs. HC-10 (IgG2a) recognized CCs devoid of a peptide. HLA-I polyreactive mAb TFL-006 (IgG2a) bound to OCs only and was inhibited by the shared peptides (¹¹⁷AYDGKDY¹²³ and ¹²⁶LNEDLRSWTA¹³⁵) found in all HLA-I isoforms.