Table 4.
HLA-I allele reactivities of the polyreactive mAbs as compared with monospecific mAbs. The values represent mean fluorescent intensities (MFIs) of the mAbs, corrected against background values. The mAbs were generated using HLA-E-recombinant heavy chains. The number of HLA antigens showing positive reactivity with the mAbs are shown in bold letters.
| mAbs | Monospecific | Polyreactive | mAbs | Monospecific | Polyreactive | mAbs | Monospecific | Polyreactive | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TFL-033 | TFL-006 | TFL-007 | TFL-033 | TFL-006 | TFL-007 | TFL-033 | TFL-006 | TFL-007 | |||
| IgG1 | IgG2a | IgG2a | IgG1 | IgG2a | IgG2a | IgG1 | IgG2a | IgG2a | |||
| Neg | 3 | 15 | 7 | B* alleles | C* alleles | ||||||
| Pos | 71 | 88 | 85 | B*0702 | 1331 | 841 | C*0102 | 7242 | 3268 | ||
| HLA-E | 24411 | 22522 | 21618 | B*0801 | 2092 | 1033 | C*0202 | 10690 | 6084 | ||
| HLA-F | 12650 | 11035 | B*1301 | 5654 | 3979 | C*0302 | 5917 | 3062 | |||
| HLA-G | 7193 | 2670 | B*1302 | 2237 | 1426 | C*0303 | 7114 | 4250 | |||
| A* alleles | B*1401 | 11319 | 8767 | C*0304 | 6584 | 3891 | |||||
| A*0101 | 2395 | 1037 | B*1402 | 4414 | 2558 | C*0401 | 2843 | 1272 | |||
| A*0201 | 856 | B*1501 | 1097 | C*0501 | 16131 | 13096 | |||||
| A*0203 | 1095 | B*1502 | 6256 | 4497 | C*0602 | 9396 | 4274 | ||||
| A*0206 | 1494 | 843 | B*1503 | 2831 | 1926 | C*0702 | 12251 | 6919 | |||
| A*0301 | 818 | B*1510 | 2616 | 1470 | C*0801 | 13456 | 10733 | ||||
| A*1101 | 10190 | 8476 | B*1511 | 9041 | 5902 | C*1203 | 5055 | 2102 | |||
| A*1102 | 860 | B*1512 | 1624 | 996 | C*1402 | 8727 | 4936 | ||||
| A*2301 | 614 | B*1513 | 5326 | 3365 | C*1502 | 6030 | 3225 | ||||
| A*2402 | 3133 | 2011 | B*1516 | 5614 | 3443 | C*1601 | 8462 | 4364 | |||
| A*2403 | 3151 | 1967 | B*1801 | 6990 | 4890 | C*1701 | 13521 | 9069 | |||
| A*2501 | 1230 | 692 | B*2705 | 2591 | 1576 | C*1802 | 17918 | 15207 | |||
| A*2601 | 3368 | 1638 | B*2708 | 4437 | 2671 | C* alleles | 0 | 16 | 16 | ||
| A*2901 | 3194 | 2256 | B*3501 | 10205 | 8594 | ||||||
| A*2902 | 2235 | 1136 | B*3701 | 6472 | 4338 | ||||||
| A*3001 | 2229 | 1237 | B*3801 | 3844 | 1820 | ||||||
| A*3002 | 3353 | 2211 | B*3901 | 7093 | 5304 | ||||||
| A*3101 | 858 | B*4001 | 5743 | 3758 | |||||||
| A*3201 | 2237 | 1508 | B*4002 | 6118 | 4675 | ||||||
| A*3301 | 2791 | 1627 | B*4006 | 15643 | 13758 | ||||||
| A*3303 | 4212 | 2961 | B*4101 | 7191 | 5277 | ||||||
| A*3401 | 6268 | 3968 | B*4201 | 636 | |||||||
| A*3402 | 1399 | 893 | B*4402 | 7062 | 4059 | ||||||
| A*3601 | 5806 | 3826 | B*4403 | 7256 | 5638 | ||||||
| A*4301 | 4420 | 2364 | B*4501 | 9535 | 7646 | ||||||
| A*6601 | 3644 | 1526 | B*4601 | 6491 | 4130 | ||||||
| A*6602 | 1395 | 789 | B*4701 | 6528 | 3895 | ||||||
| A*6801 | 1314 | 859 | B*4801 | 4365 | 2716 | ||||||
| A*6802 | 2078 | 1276 | B*4901 | ||||||||
| A*6901 | 1964 | 917 | B*5001 | 741 | |||||||
| A*7401 | 723 | B*5101 | 6205 | 3724 | |||||||
| A*8001 | 2841 | 1430 | B*5102 | 5251 | 3579 | ||||||
| A* alleles | 0 | 32 | 24 | B*5201 | 4524 | 2728 | |||||
| B*5301 | 8807 | 7323 | |||||||||
| B*5401 | 5556 | 4153 | |||||||||
| B*5501 | 2829 | 1887 | |||||||||
| B*5601 | 1386 | 777 | |||||||||
| B*5701 | |||||||||||
| B*5703 | 1229 | 600 | |||||||||
| B*5801 | 10160 | 8047 | |||||||||
| B*5901 | 5646 | 3001 | |||||||||
| B*6701 | 675 | ||||||||||
| B*7301 | 3347 | 2171 | |||||||||
| B*7801 | 6089 | 4597 | |||||||||
| B*8101 | 1352 | 729 | |||||||||
| B*8201 | 4367 | 3069 | |||||||||
| B* alleles | 0 | 48 | 44 | ||||||||