Table 5.
Evidence showed that HLA-I polyreactive mAb TFL-006 bound to OCs (β2m-free αHLA HC) but not to CCs (β2m-associated HLA heavy chains or intact HLA molecules). TFL-006 bound only with LABScreen SABs (contained both open and CCs) but not with LIFECODES, which had only CCs, as established in previous reports [45,47].
| TFL-006 (20 ug/mL) | |||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HLA-A | NC | PC | A*01:01 | A*02:01 | A*02:03 | A*03:01 | A*11:01 | A*11:02 | A*23:01 | A*24:02 | A*24:03 | A*25:01 | A*26:01 | A*29:01 | A*29:02 | A*30:01 | A*31:01 | A*32:01 | A*33:01 | A*33:03 | A*34:02 | A*36:01 | A*43:01 | A*66:01 | A*66:02 | A*68:01 | A*68:02 | A*69:01 | A*74:01 | A*80:01 | |||||||||||||
| REGULAR LABSCreen BEADSET (Lot: LS1A04-lot 10) [Closed Conformers admixed with Open Conformers] | |||||||||||||||||||||||||||||||||||||||||||
| 0 | 12 | 933 | 339 | 1018 | 193 | 4782 | 537 | 133 | 716 | 2516 | 194 | 2221 | 1017 | 778 | 1496 | 396 | 515 | 1038 | 554 | 1535 | 1353 | 2479 | 1886 | 1454 | 713 | 1185 | 3128 | 652 | 3132 | ||||||||||||||
| LIFECODES BEADSET (Lot # 3005619) (Closed Conformers only) | |||||||||||||||||||||||||||||||||||||||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||||||
| HLA-B | B*07:02 | B*08:01 | B*13:02 | B*14:01 | B*14:02 | B*15:01 | B*15:02 | B*15:03 | B*15:12 | B*15:13 | B*15:16 | B*18:01 | B*27:05 | B*27:08 | B*35:01 | B*37:01 | B*38:01 | B*39:01 | B*40:01 | B*40:02 | B*41:01 | B*42:01 | B*44:02 | B*44:03 | B*45:01 | B*46:01 | B*47:01 | B*48:01 | B*49:01 | B*50:01 | B*51:01 | B*52:01 | B*53:01 | B*54:01 | B*55:01 | B*56:01 | B*57:01 | B*58:01 | B*59:01 | B*67:01 | B*73:01 | B*78:01 | B*81:01 |
| REGULAR LABSCreen BEADSET (Lot: LS1A04-lot 10) [Closed Conformers admixed with Open Conformers] | |||||||||||||||||||||||||||||||||||||||||||
| 862 | 1226 | 2514 | 7805 | 1831 | 335 | 1935 | 1822 | 770 | 3135 | 3076 | 3096 | 634 | 1659 | 6128 | 2650 | 2521 | 704 | 3429 | 2697 | 3739 | 347 | 3650 | 1829 | 1736 | 3572 | 2152 | 3262 | 1554 | 1799 | 2461 | 2146 | 5442 | 1662 | 2519 | 3662 | 2089 | 5268 | 3553 | 406 | 1423 | 2996 | 1525 | |
| LIFECODES BEADSET (Lot # 3005619) (Closed Conformers only) | |||||||||||||||||||||||||||||||||||||||||||
| 0 | 0 | 12 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 8 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | |
| HLA-C | C*01:02 | C*02:02 | C*03:03 | C*03:04 | C*04:01 | C*05:01 | C*06:02 | C*07:02 | C*08:01 | C*14:02 | C*15:02 | C*16:01 | C*17:01 | ||||||||||||||||||||||||||||||
| REGULAR LABSCreen BEADSET (Lot: LS1A04-lot 10) [Closted Conformers admixed with Open Conformers] | |||||||||||||||||||||||||||||||||||||||||||
| 4066 | 7446 | 2458 | 4504 | 3337 | 9124 | 5644 | 8702 | 6090 | 3937 | 4465 | 4648 | 8296 | |||||||||||||||||||||||||||||||
| LIFECODES BEADSET (Lot # 3005619) (Closed Conformers only) | |||||||||||||||||||||||||||||||||||||||||||
| 1 | 20 | 0 | 0 | 8 | 24 | 4 | 97 | 1 | 0 | 4 | 0 | 10 | |||||||||||||||||||||||||||||||