Figure 2.
Cell movement effects of the 1:1 co-culture on day 4 in vitro in serum-free conditions, showing observations from individual experiments. Each plating is shown in a different color, and data are presented as mean+/−SD. (A) Frequency of spontaneous contraction. (B) Amplitude of the spontaneous contraction. (C) Time to contract at (Ci) spontaneous, (Cii) 1 Hz, and (Ciii) 2 Hz conditions. (D) Time to relax at (Di) spontaneous, (Dii) 1 Hz, and (Diii) 2 Hz. (E) This shows 50% of the contraction duration at (Ei) spontaneous, (Eii) 1 Hz, and (Eiii) 2 Hz. The comparison of each culture (hiCM, HEK–WT:hiCM and HEK-IK1:hiCM) within each experiment is shown as # in the respective experimental, and 2-way ANOVA was used for statistical analysis. The mean of all experiments was then compared using a paired t-test and this is shown as * (p < 0.05).