Table 1.
List of antibodies used for the immunofluorescent (IF) and immunohistological (IHC) analyses.
| Primary Antibody | Dilution |
Manufacturer
(Reference Number) |
| Rabbit anti-Iba1 | 1:1000 (IF) | Wako Chemicals, Richmond, VA, USA (019-19741) |
| Mouse anti-GFAP | 1:200 (IF) | Cell Signaling Technology, Beverly, MA, USA (#3670) |
| Rabbit anti-MBP | 1:2500 (IHC) | Abcam, Cambridge, UK (ab218011) |
| Rabbit anti-NfL | 1:100 (IHC) | Cell Signaling Technology, Beverly, MA, USA (#2837) |
| Mouse anti-SYP | 1:200 (IF) | Santa Cruz Biotechnology, Santa Cruz, CA, USA (sc-17750) |
| Secondary Antibody | Dilution |
Manufacturer
(Reference Number) |
| Goat anti-rabbit Alexa Fluor 594 | 1:200 (IF) | Abcam, Cambridge, UK (ab6901) |
| Rabbit anti-mouse Alexa Fluor 594 | 1:200 (IF) | Cell Signaling Technology (#4408) |
| Biotinylated goat anti-rabbit | 1:200 (IHC) | Invitrogen, Carlsbad, CA, USA (65-6140) |
Abbreviations: Iba1, ionized calcium-binding adapter molecule 1; GFAP, glial fibrillary acidic protein; MBP, myelin basic protein; NfL, neurofilament light chain; SYP, synaptophysin.