Figure 1.

Generation and selection of Nrl-expressing 661W cells. (A) RT-PCR analysis of Nrl and Rho mRNA on 661W and 661W Nrl+ cells (bulk of retroviral transduced cells). Ribosomal protein gene RPS26 (S26) was analyzed as a reference gene. Expression of Nrl and Rho was confirmed in 661W transduced cells but not 661W cells. NC: negative control without cDNA (retrotranscription −). (B) Five clones showed differential expression of rod-specific genes when analyzed by RT-PCR (RT - or +: retrotranscription − or +). S26 was analyzed as a reference gene. The primers for Nr2e3 also amplified a transcript variant with an intron inclusion reported in IMAGE:5357172. For Nr2e3, we evaluated only the lower molecular weight band corresponding to the spliced mRNA.