Figure 5.

Validation of the in vitro model for drug screening. (A) A TUNEL assay was used to determine the percentage of cell death in 661W-A11. (B) Cell viability was assessed by MTT assay. The values of cells treated with the vehicle (DMSO, control) were set as 100% cell viability. ZAP: cells treated with 400 μM zaprinast; ZAP+CN03 or CS or PEDF: cells were pretreated with 50 µM CN03 or 20 µM calpastatin peptide (CS) or 10 nM PEDF for 2 h before being stressed with 400 μM zaprinast in the presence of the neuroprotective compounds. Statistical comparison: Student’s unpaired two-tailed t-test; * p < 0.05, ** p < 0.01, *** p < 0.001.