Table 3.
Primary (A) and secondary (B) antibodies used in the immunohistochemical studies performed, showing the dilutions used and the protocol specifications.
| (A) | ||||
| Antigen | Species | Dilution | Provider | Protocol Specifications |
| NOX 1 | Rabbit | 1:250 | Abcam (ab78016) | 10 mM Sodium citrate pH = 6 before incubation with blocking solution |
| NOX 2 | Goat | 1:500 | Abcam (ab111175) | 100% Triton 0.1% in PBS, 10 min, before incubation with blocking solution |
| iNOS | Rabbit | 1:350 | Abcam (ab95866) | 10 mM Sodium citrate pH = 6 before incubation with blocking solution |
| Hif-1α | Mouse | 1:800 | Abcam (ab16066) | EDTA at pH 9 before incubation with blocking solution |
| (B) | ||||
| Antigen | Species | Dilution | Provider | Protocol Specifications |
| IgG (Mouse) |
Goat | 1:300 | Sigma-Aldrich (F2012/045K6072) |
-------------------- |
| IgG (Rabbit) |
Mouse | 1:1000 | Sigma-Aldrich (RG-96/B5283) |
-------------------- |
| IgG (Goat) |
Mouse | 1:100 | Sigma-Aldrich (A5420) |
-------------------- |