Table 2.

PCR condition for 16s RNA universal primers.

Initial Denaturation		Denaturation		Annealing		Extension		Final Extension
Temperature	Time	Temperature	Time	Temperature	Time	Temperature	Time	Temperature	Time
94 °C	5 min	94 °C	1 min	50 °C	40 s	72 °C	90 s	72 °C	5 min