Figure 2.

Curcumin reduces the degradation of primordial follicles to maintain the ovarian reserve. (A) The ovarian in vitro culture system was used for drug screening, and ovaries from 3-to-4-day-old mice were cultured for 12 days. (B,C) Follicle counting after treatment with different drugs. B shows the distribution of different stages of follicles, while C shows the number of follicles. (D) Histological changes in the ovary after curcumin treatment. In vitro experiments used P3 ovaries cultured for 7 days, and in vivo experiments used P5 mice with continuous ip injection of 100 mg/kg curcumin for 7 days. (E) Follicle counting after treatment with curcumin in vivo and in vitro. FC: follicle. * p-value < 0.05, ** p-value < 0.01, **** p-value < 0.0001 compared to the controls.