Table 2.
Discriminant metabolites from arginine, pyrimidine, purine, and tryptophan pathways. ns: Non-significant. nr: not detected or not robust feature. a Identification validated by MS/MS experiments. Significance: * p ≤ 0.05 ** p ≤ 0.01 *** p ≤ 0.001. ns: Not significant. RV: Right ventricle. LV: Left ventricle. FC: Fold Change (in green increased metabolites, in red decreased metabolites).
| Fold Change (PH/CTRL) and Significance | |||||||
|---|---|---|---|---|---|---|---|
| CH vs. Normoxia | MCT vs. Vehicle | ||||||
| Plasma | RV | LV | Plasma | RV | LV | ||
| Arginine metabolic pathway | Spermidine a | ns | 1.50 * | 1.27 *** | ns | 1.33 ** | ns |
| Acetyl-spermidine a | ns | 2.08 *** | 1.80 *** | ns | 2.97 *** | 2.02 ** | |
| Putrescine a | ns | 1.87 *** | 1.67 *** | ns | 1.75 *** | ns | |
| Guanidinoacetate a | 1.68 *** | 3.01 *** | 1.97 *** | 0.74 * | 1.54 * | ns | |
| Arginine a | 0.75 * | 0.85 * | 0.81 *** | 0.76 ** | 0.85 ** | ns | |
| Creatine a | 0.72 ** | 0.79 *** | 0.77 *** | ns | 0.88 ** | 0.91 *** | |
| Fumarate a | 1.55 * | ns | 1.23 *** | ns | 1.43 * | ns | |
| Ornithine a | 1.34 *** | ns | ns | ns | 1.57 ** | ns | |
| Creatinine a | 1.15 *** | ns | ns | 1.17 ** | ns | ns | |
| Aspartate a | 1.40 ** | 1.26 * | ns | ns | ns | ns | |
| Oxoglutarate a | 1.56 *** | 0.75 * | ns | ns | ns | ns | |
| Guanidinobutanoate a | 1.94 *** | ns | 1.15 * | ns | ns | ns | |
| N-acetyl-glutamate a | 1.58 ** | ns | ns | ns | ns | 1.5 * | |
| Glutamine a | 1.36 *** | ns | ns | 0.89 * | 0.86 ** | ns | |
| Citrulline a | ns | ns | 0.88 * | 0.87 * | 0.85 * | 0.80 ** | |
| Aminobutanoate a | ns | ns | 0.89 * | ns | ns | ns | |
| Glutamate a | ns | ns | 0.90 * | ns | ns | ns | |
| Trans-4-L-hydroxy-proline a | 1.1 * | ns | 0.85 * | ns | ns | ns | |
| Pyrimidine metabolic pathway | Orotate a | 1.87 ** | 2.13 *** | 2.05 *** | ns | 2.18 ** | ns |
| Pseudouridine a | 1.20 *** | 1.27 ** | 1.19 * | 1.09 * | 1.28 ** | 1.29 * | |
| Uridine a | 1.50 * | 1.25 * | ns | ns | 1.26 * | ns | |
| Uracil a | 1.31 * | ns | 1.25 ** | 1.76 * | 1.28 ** | ns | |
| Thymidine a | 1.60 *** | ns | ns | 1.45 ** | 1.25 * | ns | |
| Thymine a | 1.30 ** | ns | ns | 1.51 * | 1.47 ** | ns | |
| Deoxyuridine a | 1.39 *** | ns | 1.24 *** | ns | 1.41 * | ns | |
| Dihydrouracil a | 1.28 ** | ns | ns | 1.66 ** | ns | ns | |
| Cytosine a | 1.27 ** | 1.19 ** | 1.18 * | ns | 1.19 * | ns | |
| Deoxycytidine a | 1.32 *** | 1.20 * | 1.28 ** | ns | ns | ns | |
| Acetyl-cytidine a | ns | ns | ns | 1.37 * | 1.64 ** | 1.49 * | |
| 5-Methyldeoxycytidine a | ns | 1.45 * | 1.55 ** | ns | 1.59 ** | ns | |
| Dihydroorotate | ns | ns | ns | 1.60 * | ns | ns | |
| Purine metabolism | Allantoin a | 1.21 ** | 1.24 * | 1.39 *** | 1.21 * | 1.26 ** | 1.34 * |
| 7-Methyl-guanine a | 1.18 * | 1.23 * | 1.24 ** | ns | 1.46 ** | 1.51 * | |
| Methyl-inosine a | 1.23 * | ns | ns | ns | 1.46 * | ns | |
| Xanthosine a | nr | 1.38 * | ns | nr | 1.90 ** | ns | |
| Xanthine a | nr | ns | 1.12 * | nr | 1.18 * | ns | |
| Inosine a | nr | ns | 0.92 *** | nr | 0.90 ** | 0.94 * | |
| Guanosine a | nr | ns | 0.85 * | nr | 0.71 *** | 0.82 * | |
| Adenine a | nr | 0.67 * | ns | nr | ns | ns | |
| 5’AMP a | nr | 1.18 * | ns | nr | ns | ns | |
| Urate a | ns | ns | 1.28 * | 2.50 * | 1.8 * | ns | |
| Tryptophan metabolic pathway | Indole-propionate a | 1.60 * | 1.81 * | 2.11 ** | ns | ns | ns |
| Indolelactate a | 0.55 *** | 0.65 * | ns | ns | ns | ns | |
| Kynurenine a | 1.28 * | ns | 1.34 * | ns | ns | ns | |
| Tryptophan a | ns | ns | 1.15 * | ns | 1.32 ** | ns | |
| Tyrosine a | ns | ns | ns | 1.33 * | 1.34 ** | 1.21 * | |
| Phenylalanine a | 0.82 *** | ns | ns | ns | 1.28 * | ns | |
| Acetyl-serotonin | ns | ns | ns | 0.57 ** | 1.54 ** | 1.88 * | |
| Serotonin | 0.63 * | nr | nr | ns | nr | nr | |
| Xanthurenate a | ns | nr | nr | 1.39 * | nr | nr | |
| Aminophenol | ns | ns | ns | 0.59 * | ns | ns | |
| Kynurenate a | ns | ns | ns | 1.58 * | ns | ns | |
| N-acetyl-tryptophan a | ns | ns | ns | 1.51 * | ns | 2.47 * | |