Figure 9.

The schematic of self-designed distraction device. It is developed based on an inverted microscope for the purpose of real-time observation. Being mounted over the objective is an optical protrusion, on the top of that is a silicone membrane, holding hBMSCs in a culture well. There was cyclic force pressing (to position 2) or lifting (to position 1) the culture well sinusoidally, which made the silicone membrane stretched on the optical protrusion. The distraction was then transducted from the membrane into the intracellular parts of hBMSCs.