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. 2021 Jun 26;22:480. doi: 10.1186/s12864-021-07640-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Immuno-localization pattern of the two CA-BDs viz. BuBD-126 and 129 using the in house generated anti-BuBD-129 and 126 antibodies, respectively in rabbit against selected B-epitopes. The decrease of the fluorescent signal intensity pertaining to the removal of the CA-BDs, BuBD-126 and 129 from the buffalo bull sperm surface is observable after both, the 2x-DPBS and PI-PLC treatments