Table 1.

The seven treatment sub-groups for sperm-surface protein extraction from two treatment classes and the corresponding TPP results indicating total spectra, correctly and incorrectly identified proteins at p ≥ 0.6 and 0.99

Sample	PEP-XML Total spectra	Ipro.pep.xml Total spectra	Prot.xml Total proteins	Total proteins with p ≥ 0.6	Incorrectly identified with iProphet p ≥ 0.6	Total proteins with p ≥ 0.99	Incorrectly identified with iProphet p ≥ 0.99
2x-DPBS-30 min	26,535	7252	1733	875	76	317	0
4x-DPBS-30 min	26,470	8399	1725	956	76	391	0
2x-DPBS-60 min	25,947	7742	1898	1013	90	395	0
4x-DPBS-60 min	25,951	8210	2162	1244	99	432	0
1 U/ml	24,829	6557	1788	992	99	385	0
1.5 U/ml	24,587	6343	1932	1005	76	353	0
2 U/ml	24,881	5978	1662	793	63	364	0