Fig. 3.

BTKi inhibit chemotactic migration in MCL cells. a Chemotaxis of JeKo-1, Mino and primary MCL cells (n = 3, primary MCL cells collected from patient 1–3.) was measured by a Transwell assay measuring migration towards either medium or stroma-conditioned medium for 8 h. Data are shown as mean ± SEM; *P < 0.05; **P < 0.01, as calculated using the Student’s t-test. b Heatmap of chemotaxis-related DEGs regulated by Ibrutinib, Acalabrutinib or Zanubrutinib in both JeKo-1 and Mino cells, versus control respectively. Red represents upregulation and blue indicates downregulation. c CCL3 and CCL4 levels were examined in the supernatants of JeKo-1, Mino and primary MCL cells (n = 3, primary MCL cells collected from patient 1–3.) treated with individual BTKi (2 μM) for 48 h compare to untreated cells in the presence of anti-human IgM F (ab)₂. Data are shown as mean ± SEM; **P < 0.01; ***P < 0.001, as calculated using the Student’s t-test