Fig. 3.

Cell-free synthesis of capsid protein derivatives of common vole HEV (cvHEV), HEV-2 (gt2a) and HEV-4 (gt4a), and detection by Western blot analysis. (a) Binding of G117-AA4 antibody to cell-free synthesized cvHEV ORF2-encoded proteins. (b) Autoradiography of ¹⁴C-labelled cvHEV protein visualized on the blot. Constructs with (Mel +) and without (Mel -) the Mel-signal peptide. Proteins were synthesized in CHO and Sf21 lysates. Proteins were blotted in a native (+) and precipitated (-) form. (c) Binding of G117-AA4 antibody to cell-free synthesized cvHEV, HEV-2 (gt2a) and HEV-4 (gt4a). (d) Autoradiography of ¹⁴C-labelled proteins, initially detected by the monoclonal G117-AA4 antibody, visualized on the blot. (e) Western Blot reactivity of polyclonal rabbit serum with cell-free synthesized cvHEV, HEV-2 (gt2a) and HEV-4 (gt4a). (f) Autoradiography of ¹⁴C-labelled proteins, initially detected by the polyclonal serum visualized on the blot. NTC, no template control