Table 1.
Sequences of primers used for construction and sequencing of HEV-3, rat HEV and bat HEV capsid protein derivatives. italic, restriction site KpnI/XbaI; bold, viral sequence
| Target | Strain | Designation of oligonucleotide | Sequence of oligonucleotide | Reference |
|---|---|---|---|---|
| HEV-3 | wbGER27 | wbCtr fw | 5’-TATATCTAGAACAATGACAGCCCGTCATCGGCTGCGCCGCGGTGCTG-3’ | Dremsek et al. 2012 |
| wbCtr rev | 5’-TATATCTAGATTATGCAAGAGCCGAATGTGGGGCTAAAACAAC-3’ | |||
| rat HEV | R4 | rCtr fw | 5'-TATATCTAGAACAATGACAGCCCCGCATAAGATCAAGCGGCT-3' | Dremsek et al. 2012 |
| rCtr rev | 5'-TATATCTAGATTACTGCTCAGTCGGGCTGGGGCCGATA-3' | |||
| rHE_fw_Ctr | 5'-TCAGATCTGCATGCGGTACCAGCCCCGCATAAGATCAAG-3' | This study | ||
| rHE_re_Ctr | 5'-GCAGGTCGACCCGGGGTACCTTACTGCTCAGTCGGGCTGG-3' | |||
| rHE_re_F1 | 5'-GCAGGTCGACCCGGGGTACCTTAGCGAGCGGGCGCAGGG-3' | |||
| rHE_fw_F3 | 5'-TCAGATCTGCATGCGGTACCAGAGTATGCCCAATCGCAG-3' | |||
| bat HEV | batHEV/BS7/GE/2009 | bF1 fw | 5'-TATAGGTACCAACTCAGCACCGCCTGACCCGCGGG-3' | This study |
| bF1 rev | 5'-TATAGGTACCTTAACGCTTCGGGGCAGGCGCAGGTGA-3' | |||
| pQE | - | pQE_insert_fw | 5'-AGGAATTGAAAGTGACACG-3' | Qiagen |
| pQE | - | pQE_insert_rev | 5'-ATTACTGGATCTATCAACAGG-3' | Qiagen |