Overall mapping of all tsRNAs on a tRNA length scale revealed the preferential loci from which tsRNAs are derived from the mature full tRNA under traditional protocol and different enzymatic treatments. a, mouse brain, (b) mouse liver, (c) mouse spleen, (d) mouse mature sperm, (e) mouse MEFs (day 0), (f) mouse intermediate cells (day 3), (g) mouse iPSCs, (h) human HeLa cells, (i) human hESCs-primed, (j) mouse mature sperm heads, and (k) human hESCs-naïve. Mapping plots are presented as mean ± SEM.