Table 2.

Summarizing features of the 3 splicing analyses tools

	rMATS	MISO	SUPPA2
Version	4.0.2	0.5.4	2.3
Release Date	May 2018	July 2017	February 2018
Other required tools	None	Samtools	Kalisto/Salmon
Reference	GTF	GFF3	GTF
Input file	FASTQ/BAM	BAM	TPM
Supported experiments	Two groups, paired	1 versus 1 comparison	Two groups, paired
Job time	++	– –	+
RAM	+ +	– –	+ +
CPU	+ +	– –	+ +
Ease of use	+ +	– –	–
FPGS 8PR detection	+/−	+	+/−

 ++ , very good; + , good;  +/− , average; –, poor; ––, very poor