a, As in Fig. 1a, but a side view additionally showing a 6-Å-lowpass-filtered map at a lower contour level (semitransparent surface). b, As in a, but showing a view from cytosol. The anchor domain of Sec62 is indicated by a dotted oval. c, As in b, but showing the map of the Sec62+/plug-open class of TlSec. Semitransparent surface is a 7-Å-lowpass-filtered map. d, Yeast growth complementation tests for Sec62 anchor mutants. The yeast strain (ySI62) whose endogenous Sec62 is expressed under a tetracycline-repressible promoter was transformed with a CEN/ARS plasmid expressing WT or indicated mutant Sec62 under its native promoter. As a control, empty vector was used. In the right panels, 10 μg/mL doxycycline was included to repress the expression of endogenous Sec62. All growth assays were performed at 30°C. The top two panels (single and double mutants) were grown on synthetic complete (SC) medium lacking leucine, and the bottom two panels (triple and quadruple Ala mutants) were grown on YPD medium. e, As in Extended Data Fig 2a, but with the Δanchor mutant of TlSec. f, As in Extended Data Fig. 2e, but showing the 4.4-Å-resolution map of Δanchor TlSec. Semitransparent surface, 7-Å-lowpass-filtered map at a lower contour level. We note that the conformation of Δanchor TlSec is essentially identical to ΔSec62 TlSec. An uncropped image for panel e is available as source data.