FIGURE 2.

Biofilm formation of Bacillus subtilis WT and single ∆rap‐phr mutants. Overnight cultures of B. subtilis WT and ∆rap‐phr mutants were spotted on MSgg medium solidified with 1.5 % agar (left) or inoculated in MSgg with a starting OD₆₀₀ = 0.05 (right). Images were taken from above after 48 h of incubation at 30°C using a stereomicroscope. Letters denote the corresponding ∆rap mutant, that is, a = ∆rapA, b = ∆rapB, and so forth, but l = ΔrapP. Bar denotes 5 mm for the biofilm colony images